褪黑素(MT)检测试剂盒(酶联免疫吸附试验法,小样本)

Mini Samples ELISA Kit for Melatonin (MT)

N-Acetyl-5-Methoxytryptamine

  • 褪黑素(MT)检测试剂盒(酶联免疫吸附试验法,小样本) 产品包装(模拟)
  • 褪黑素(MT)检测试剂盒(酶联免疫吸附试验法,小样本) 产品包装(模拟)
  • 褪黑素(MT)检测试剂盒(酶联免疫吸附试验法,小样本) 实验结果图
  • MEA908Ge.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测褪黑素(MT)检测试剂盒(酶联免疫吸附试验法,小样本),经检测与其它相似物质无明显交叉反应。
由于受到?#38469;?#21450;样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的褪黑素(MT)检测试剂盒(酶联免疫吸附试验法,小样本)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 93-101 97
EDTA plasma(n=5) 98-105 101
heparin plasma(n=5) 89-96 92

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、?#23567;?#39640;值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、?#23567;?#39640;值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的褪黑素(MT)检测试剂盒(酶联免疫吸附试验法,小样本),并倍?#35748;?#37322;成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中褪黑素(MT)检测试剂盒(酶联免疫吸附试验法,小样本)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 79-99% 78-98% 84-105% 81-101%
EDTA plasma(n=5) 78-99% 96-105% 78-102% 95-103%
heparin plasma(n=5) 85-93% 98-105% 94-101% 85-99%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环?#31243;?#20214;需尽量保持一致,尤其是实验室内温?#21462;?#28287;度及温育条件。其次由同一实验员来进行操作可减少?#23435;?#35823;差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)25µL后,立即加入检测溶液A25µL,
    37°C孵育1小时;
3. 甩干,洗板3次;
4. 加检测溶液B50µL,37°C孵育30?#31181;櫻?br/> 5. 洗板5次;
6. 加TMB底物50µL,37°C孵育10-20?#31181;櫻?br/> 7. 加终止液25µL,立即450nm读数。

实验原理

本试剂盒应用竞争?#31181;?#37238;联免疫分析法测定标本中待测物质水平。将褪黑素(MT)检测试剂盒(酶联免疫吸附试验法,小样本)单克隆抗体包被微?#35013;澹?#21046;成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结?#31232;?#28201;育后经洗?#23588;?#25481;未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到?#31181;疲?#26174;色愈浅。显色的深浅与酶量?#25910;?#30456;关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓?#21462;?

相关产品

编号 适用物种:Pan-species (General,通用) 应用(仅供研究使用,不用于临床诊断!)
CPA908Ge11 褪黑素(MT)牛血清白蛋白?#21058;?#29289; Immunogen; SDS-PAGE; WB.
CPA908Ge21 褪黑素(MT)卵白蛋白?#21058;?#29289; Immunogen; SDS-PAGE; WB.
PAA908Ge01 褪黑素(MT)多克隆抗体 WB; IHC; ICC; IP.
MEA908Ge 褪黑素(MT)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA908Ge 褪黑素(MT)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA908Ge 褪黑素(MT)检测试剂盒(酶联免疫吸附试验法,快捷型) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA908Ge11 褪黑素(MT)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

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Experimental Gerontology Improvement of oxidative stress and immunity by melatonin: An age dependent study in golden hamster [Pubmed: 23220117]
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